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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in marketing mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually explained. Two extra splice variants were being described but in depth nucleotide sequence information will not be nevertheless available.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guide outlines more probable optimization of mobile and immunoassay parameters to be certain the absolute best final results are...
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This tutorial outlines further achievable optimization of mobile and immunoassay parameters to make certain the absolute best results are...
Antibodies certain to the blocking peptide no longer bind on the epitope about the target protein. By evaluating the staining from your blocked antibody vs . the antibody by yourself, you can see which staining is precise.
Concentrate on Facts Cyclin E belongs to your remarkably conserved cyclin household, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and capabilities to be a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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